Submitted by: Kris Raisanen Schourek

Subject and grade level: AP biology, 11 and 12th grades

Overview: I intend to adapt the Richard E. Lee Jr. experiment ³Using Microrespirometers to Measure O2 Consumption by Insects and Small Invertebrates² to enhance my newly developed unit on respiration.

Purpose: To provide a hands-on experience with the gas exchanges involved in cellular respiration which providing an opportunity to observe the impact of assorted variables on the rate of respiration.

Objectives: 1. Oxygen is taken in and carbon dioxide is released during cellular respiration. 2. A variety of variables impact the rate at which cellular respiration occurs.

Resources/Materials: Using Microrespirometers to Measure O2 Consumption by Insects and Small Invertebrates: by Richard E. Lee, Jr. Syringes, micropipettes, aquarium tubing, fabric tape, soda lime, small invertebrates, small plants and sprouting seeds.

Activities and Procedures:

1. 1. Construct microrespirometers.
2. Discuss possible variables that may impact the rate of cellular respiration. Examples: Species of invertebrate, mass of invertebrate, body temperature of invertebrates, present or absence of lightŠ and others to be suggested by students.
3. Each lab group writes its own experimental procedure and materials list.
4. Each lab group carries out its own series of experiments.
5. Lab reports are written.
6. Lab reports are evaluated.
7. General class discussion of results.

Tying it all together: In the final class discussion, students can share results and reflections about their procedures and results. Questions, answers, and feedback will be encouraged. Core knowledge as well as sound scientific method will be reinforced.

Assessment: Each lab group will write a formal lab report, following the prescribed format. It will include PROBLEM, PROCEDURE, INDEPENDENT VARIABLE, DEPENDENT VARIABLE, CONSTANTS, OBSERVATIONS, DISCUSSION (questions will be provided) and conclusion. Each lab report will be individually evaluated.

Standards Addressed: I. Constructing New Scientific Knowledge. II. Reflecting on Scientific Knowledge. III. Using Scientific Knowledge in Life Science 1.Cell